Senior Associate Scientist – Fluid Biomarker Assay Development

Senior Associate Scientist – Fluid Biomarker Assay Development

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Job Description

The Fluid Biomarkers group (Section of Clinical Sciences) is seeking a creative and collaborative lab-based scientist engaged in targeted proteomics for the detection of proteins and metabolites in different biological matrices (including plasma, serum, CSF, urine, tissues. This role is required to develop and implement clinically robust LC-MS assays to support clinical programs across the Neurology portfolio. The successful candidate will be capable of multitasking and effective planning to consistently meet deadlines.

• Targeted protein biomarker assay development using state of the art liquid-chromatography-mass spectrometry (LC-MS) platforms including nano-LC, orbitrap (Q-Exactive, Q-Exactive +, Q-Exactive HF/HFX, Fusion) and UPLC-QQQ MS (AB Sciex)

  • Sample preparation (tissues and cell lysates) for protein quantification
  • Biotinylation and various bead-antibody conjugation techniques
  • Identification and selection of antibodies for immunoprecipitation prior to LC-MS analysis
  • Identification of novel techniques and strategies to enhance assay sensitivity
  • Fit for purpose qualification of protein and small molecule LC-MS assays
  • LC-MS instrumentation troubleshooting

• Point of contact with Contract Research Organizations
  • Work with CRO partners to oversee biomarker assay implementation including assay interpretation

• Timely recording keeping and data entry into Electronic Lab Notebook
• Prepare, review, and deliver scientific presentations for internal/external use

Qualifications

• Proven experience with LC-MS instrumentation is required with experience in targeted and global proteomic profiling.
• Preference will be given to candidates with hands on experience in developing and qualifying high resolution (Orbitrap) LC-MS assays, including immunoprecipitation LC-MS for the detection of proteins in biological samples
• Expertise using Skyline and familiarity with Thermo Xcalibur, Proteome Discoverer, AB Sciex Analyst
• Excellent assay troubleshooting skills
• Familiarity with neuroscience, including knowledge of neurodegenerative diseases (AD, PD, ALS) is considered a plus but not required
• Experience in or willingness to learn classic immunoassay based assays (ELISA)
• Demonstrated track record of providing support activities for multiple research projects
• Solid work ethic and demonstrated record of productivity such as participating in peer reviewed publications or scientific poster presentations

• BS with 10+ years experience or MS with 7+ years experience in relevant scientific area

Level of the position will be commensurate with qualifications and experience.

All your information will be kept confidential according to EEO guidelines.

Additional Information

The Fluid Biomarkers group (Section of Clinical Sciences) is seeking a creative and collaborative lab-based scientist engaged in targeted proteomics for the detection of proteins and metabolites in different biological matrices (including plasma, serum, CSF, urine, tissues. This role is required to develop and implement clinically robust LC-MS assays to support clinical programs across the Neurology portfolio.