Req #: 207563 Department: DEPARTMENT OF LABORATORY MEDICINE AND PATHOLOGY Posting Date: 06/01/2022 Closing Info: Closes On 06/08/2022 Salary: Salary is commensurate with experience and education. Limited Recruitment: Open to Department employees only Shift: First Shift Notes: As a UW employee, you will enjoy generous benefits and work/life programs. For detailed information on Benefits for this position, click here. (https://hr.uw.edu/benefits/wp-content/uploads/sites/3/2018/02/benefits-professional-staff-librarians-academi-staff-20210208.pdf) As a UW employee, you have a unique opportunity to change lives on our campuses, in our state and around the world. UW employees offer their boundless energy, creative problem solving skills and dedication to build stronger minds and a healthier world. UW faculty and staff also enjoy outstanding benefits, professional growth opportunities and unique resources in an environment noted for diversity, intellectual excitement, artistic pursuits and natural beauty. The University of Washington, Department of Laboratory Medicine and Pathology is a broad and dynamic department that strives for a consistent level of high achievement in all of its activities. The Department offers full diagnostic services in all areas of Laboratory Medicine and Pathology, is a top ranked research organization as measured by research awards from the National Institutes of Health and offers an array of specialized educational training programs. The UW Medicine Department of Laboratory Medicine and Pathology brings together state-of-the-art technology and highly trained, world renowned personnel to provide comprehensive diagnostic and personalized service to physicians, community hospitals, independent laboratories, and other health care organizations. Our operations are based at the University of Washington Medical Center, Harborview Medical Center, South Lake Union, Northwest Hospital and the Seattle Cancer Care Alliance. Faculty members also serve at Seattle Children's Hospital, the Veteran’s Administration Puget Sound Healthcare System, and the Fred Hutchinson Cancer Research Center. These collaborative efforts have generated innovative, translational studies in the basic mechanisms of disease as they apply to diagnostic laboratory medicine and pathology. The UW BioRepository and Integrated Neuropathology (BRaIN) Research Laboratory has an outstanding opportunity for a Full Time Research Scientist 3. This is an excellent opportunity for the scientist to participate in a team whose goal is to support human brain research from initial donation to state-of-the-art cell and molecular tissue research. This position will lead complex procedures for services such as human brain collection, dissection, stabilization, and processing, as well as tissue and fluid-based integrated qualitative and quantitative histological and immunoassays and downstream analytical approaches including solution-phase quantitation and digital image analysis. RESPONSIBILITIES: Lead BRaIN lab activities associated with research donor brain collection, dissection, preservation, and analysis and will manage an on-call team for these cases; Participate in lab and other meetings, provide regular progress updates on research data collection, review work lists and prioritize experiments in conjunction with the PI and interact with the research team and collaborators. Lead the approach to digitization of 3D scanned images and transfer of files to collaborators Lead histological tissue processing and independently design research approaches as needed for numerous large quantitative studies and experiments requiring histological and antibody-based tissue section staining for evaluation across human and animal disease models; Perform special stains via automated stainer and manual staining; maintain and supervise all histological laboratory instruments; perform quality control and quality assurance; develop new histological procedures and research approaches for ongoing and future studies; monitor reagent use, and perform preventative maintenance on instruments according to prescribed protocols. Participate in quantitative tissue-based assays, including tissue dissection and preparation of extractions for these assays, which include protein measurements and targeted and multiplexed immunoassays to determine molecular and/or spatial transcriptomic changes associated with neurodegeneration, neurotrauma, and dementia Participate in/provide support for other laboratory projects, which may include tissue clearing and staining for three dimensional microscopic reconstructions of brain injury and disease, in silico 3D reconstructions of microscopic and gross brain images, participation in slide scanning and image analytics, tissue processing for analysis by mass spectrometry and chromatography techniques, and other tissue analytics; Maintain updated records and protocols in the lab, and will interface with other senior research scientists and the PI, particularly with respect to methods components for manuscript preparation. Assist with a variety of special projects, and research related duties as assigned in a research environment where innovation and novel analytic applications form the foundation of an integrated model of precision neuropathology. This position has operational access to patient health information and clinical materials including pathology specimens and archival blocks and slides, and for these activities must adhere to the covered entity HIPPA Privacy and CLIA rules and has 'dotted line' reporting to the CLIA certificate holders and applicable clinical laboratory management staff. MINIMUM REQUIREMENTS: A Bachelor's Degree with coursework in chemistry, biology and other related biological sciences. Experience may substitute for education. Minimum of 3 years’ experience with the following: Immunohistochemistry, immunofluorescence staining, team management Equivalent education/experience will substitute for all minimum qualifications except when there are legal requirements, such as a license/certification/registration. DESIRED: HT or HTL certified by the American Society of Clinical Pathologist (ASCP) Prior clinical anatomic laboratory experience Experience with the following: Immunohistochemistry, immunofluorescence staining, protein assays and solution phase immunoassays, molecular applications including tissue and single cell RNA and DNA sequencing, brightfield or fluorescence microscopy. Conditions of Employment: UW Research Scientists are essential to BRaIN research laboratory activities; this position requires work after-hours, weekends and or outside normal business hours. Application Process: The application process for UW positions may include completion of a variety of online assessments to obtain additional information that will be used in the evaluation process. These assessments may include Work Authorization, Cover Letter and/or others. Any assessments that you need to complete will appear on your screen as soon as you select “Apply to this position”. Once you begin an assessment, it must be completed at that time; if you do not complete the assessment you will be prompted to do so the next time you access your “My Jobs” page. If you select to take it later, it will appear on your "My Jobs" page to take when you are ready. Please note that your application will not be reviewed, and you will not be considered for this position until all required assessments have been completed. University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to, among other things, race, religion, color, national origin, sexual orientation, gender identity, sex, age, protected veteran or disabled status, or genetic information.