Reference #: 1010329
SHIFT:Day (United States of America)Seeking Breakthrough MakersChildren's Hospital of Philadelphia (CHOP) offers countless ways to change lives. Our diverse community of more than 20,000 Breakthrough Makers will inspire you to pursue passions, develop expertise, and drive innovation.At CHOP, your experience is valued; your voice is heard; and your contributions make a difference for patients and families. Join us as we build on our promise to advance pediatric care-and your career.CHOP's Commitment to Diversity, Equity, and InclusionCHOP is committed to building an inclusive culture where employees feel a sense of belonging, connection, and community within their workplace. We are a team dedicated to fostering an environment that allows for all to be their authentic selves. We are focused on attracting, cultivating, and retaining diverse talent who can help us deliver on our mission to be a world leader in the advancement of healthcare for children.We strongly encourage all candidates of diverse backgrounds and lived experiences to apply.A Brief Overview
The Maris lab seeks a dedicated research technician to integrate into the large collaborative laboratory focused on developing new immunotherapies for children with cancer. We seek a highly motivated individual interested in growth as a scientist over time and passionate about improving outcomes for patients with childhood solid tumors.An entry levelResearch Technician IIprimary responsibility willbe the management of the lab's central stock of neuroblastoma cell lines. This will include thawing and providing other lab members with requested cell lines in a timely manner while maintaining proper sterile cell culture technique. A Research Technician will need to manage a detailed online record to ensure an ample supply of cryovials for each cell line in case lab members or collaborators request an aliquot. As part of maintaining the neuroblastoma cell lines for the lab an applicant will be required to perform rigorous quality control tests to ensure the integrity of the cell lines for fellow researchers in the lab. These tests include screening every cell line at thaw for any potential traces of mycoplasma as well as extracting DNA from each line when freezing down aliquots to add to the cell bank. These DNA samples will then be analyzed to determine the short tandem repeats (STR) of the DNA. This will then be compared to a verified STR profile of our neuroblastoma cell lines to ensure the identity of the lines frozen down.Along with maintaining the integrity of the cell bank a Research Technician will be required to work alongside other researchers in the lab on a research project, where they will be required to independently run experiments then analyze and interpret the data and present data in collaborativeweekly lab meetings. These experiments may include techniques such as Western blotting, ELISA, PCR, DNA/RNA extraction,flow cytometry, andcytotoxicity assays utilizing both the IncuCyte and RT-CES instruments. An applicant will be required to assist in other lab chores such as shipping research material to collaborators both domestically and internationally.What you will do
Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
Performs research procedures on experimental samples as directed
Records and checks experiments/ results for completeness and accuracy
Performs calculations and maintains appropriate log and lab notebooks
Performs tasks that require familiarity with fundamental techniques and lab equipment
May assist with planning specific research protocols and procedures
Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
Assists in care and maintenance of lab equipment
May coordinate schedules of tests and procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
Conducts literary searches as directed
Education Qualifications
High School Diploma / GED Required
Associate's Degree Preferred
Bachelor's Degree Preferred
Experience Qualifications
At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Required
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Preferred
Skills and Abilities
Intermediate understanding of lab procedures, protocols, and equipment.
Basic exposure to lab specific experiments / techniques and scientific analysis
Excellent verbal and written communications skills
Excellent organizational skills
Solid critical thinking / problem-solving skills
Solid analytical skillsTo carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must be fully vaccinated against COVID-19 and receive an annual influenza vaccine. Learn more.Employees may request exemptions for valid religious and medical reasons. Start dates may be delayed until candidates are immunized or exemption requests are reviewed.EEO / VEVRAA Federal Contractor | Tobacco StatementChildren's Hospital of Philadelphia is an equal opportunity employer. We do not discriminate on the basis of race, color, gender, gender identity, sexual orientation, age, religion, national or ethnic origin, disability or protected veteran status.
