Laboratory SpecialistPosition is currently available for a Laboratory Specialist within the Vascular Medicine Institute of the University of Pittsburgh, Department of Medicine. The ideal candidate should have extensive experience with management of mouse colony, mouse genotyping using PCR and gel electrophoresis. Experience with standard molecular biology and biochemistry techniques is highly desired including Western Blot, DNA-gel electrophoresis, RT-PCR, immunohistochemistry, co-immunoprecipitation, ELISA, cell transfection, etc. Prior work with mouse models of disease and genotyping of mice is preferred. Experience in performing tail vein and IV injections in mice are highly desirable. Excellent verbal and written skills, attention to detail, and precise record keeping is expected. Cell culture experience, including human and rodent endothelial cells, is highly appreciated. Duties include management of mouse colony and genotyping, running biochemical assays, ordering lab supplies, and record keeping.Main Duties:Manage mice colonies, breeding according to investigators' needs, caring for animals, and maintaining their health and well-being.Perform wide variety of biomolecular assays including western blot, PCR, immunoprecipitation, cell culture, ELISA, and immunoassay if needed.Perform complex animal procedures including tail snips and blood collection for genotyping and other more advanced genotyping techniques.Provide weekly reports on size and status of each colony, assisting in reconciling animal-related accounts, and other duties as assigned.Ordering and management of lab supplies including reagents and mice. Also management of IACUC protocols including modification and submission of new protocols.Job SummaryPerforms a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.Essential FunctionsProcess data and use computer keyboard. Accurate visual recognition of small samples and repetitive pipetting; handling mice requires accurate eye to hand coordination, excellent visual acuity, and manual dexterity. Must be very organized and be able to keep a database for mouse colonies, biological specimens, reagents, chemicals, etc. Must be able to reach/stack consumables on shelves.Physical EffortThis position will require standing for long periods of time and/or walking for extending periods (if resources need to be accessed at other University labs).The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.Assignment Category: Full-time regular Job Classification: Staff.Laboratory Research Technician II Job Family: Research Job Sub Family: Laboratory Campus: Pittsburgh Minimum Education Level Required: Bachelor's Degree Minimum Years of Experience Required: 1 Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement. Work Schedule: Mon-Fri Standard Hours Work Arrangement: Mon-Fri Standard Hours Hiring Range: TBD Based Upon Qualifications RelocationOffered: No Visa Sponsorship Provided: No Background Check: For position finalists, employm Equal Employment Oppo tunity/M/F/disability/protected veteran status PI241028209
